{"id":1193,"date":"2011-06-16T13:44:37","date_gmt":"2011-06-16T11:44:37","guid":{"rendered":"http:\/\/www.tourdebier.cz\/francek\/?p=1193"},"modified":"2015-07-20T20:56:33","modified_gmt":"2015-07-20T18:56:33","slug":"kvasinky-v-solnem-nalevu","status":"publish","type":"post","link":"https:\/\/www.tourdebier.cz\/francek\/kvasinky-v-solnem-nalevu\/","title":{"rendered":"Kvasinky v soln\u00e9m n\u00e1levu"},"content":{"rendered":"

I kdy\u017e se zd\u00e1, \u017ee skladov\u00e1n\u00ed kvasinek na agaru v d\u00e9lce cca 3-6 m\u011bs\u00edc\u016f je dostate\u010dn\u00e9, tak p\u0159i dr\u017een\u00ed v\u00edce kvasni\u010dn\u00fdch kmen\u016f se dostaneme do situace, kdy mus\u00edme kvasinky p\u0159eo\u010dkov\u00e1vat, co\u017e nemus\u00ed b\u00fdt a\u017e tak pravideln\u011b z\u00e1bavn\u00e9. Proto jsem hledal zp\u016fsob, jak dobu skladov\u00e1n\u00ed prodlou\u017eit. Po p\u0159e\u010dten\u00ed n\u011bkolika zm\u00ednek na zahrani\u010dn\u00edch webech o mo\u017enosti ukl\u00e1d\u00e1n\u00ed kvasinek do fyziologick\u00e9ho roztoku ( 0,9%n\u00ed roztok NaCl ve steriln\u011b upraven\u00e9 H2O) jsem i j\u00e1 tuto metodu vyzkou\u0161el a zd\u00e1 se, \u017ee je to ta prav\u00e1 metoda pro moje dom\u00e1c\u00ed pou\u017eit\u00ed. Fyziologick\u00fd roztok slou\u017e\u00ed pro\u00a0nastaven\u00ed \u00a0osmotick\u00e9 rovnov\u00e1hy mezi kvasinkov\u00fdmi bu\u0148kami a vn\u011bj\u0161\u00edm prost\u0159ed\u00edm, kter\u00e9 bu\u0148ku obklopuje. \u017diviny z\u016fst\u00e1vaj\u00ed v bu\u0148ce a postupn\u011b se spot\u0159ebov\u00e1vaj\u00ed, tak\u017ee kvasinku vyhladov\u00edme a ulo\u017e\u00edme ke sp\u00e1nku.<\/p>\n

Metoda je :<\/strong><\/p>\n

    \n
  1. jednoduch\u00e1<\/li>\n
  2. levn\u00e1<\/li>\n
  3. uskladn\u011bn\u00ed\u00a0\u00a0 v\u00a0\u0159\u00e1du rok\u016f – pr\u00fd (m\u00e1 zku\u0161enost –\u00a0v\u00edce ne\u017e rok)<\/li>\n<\/ol>\n

    \u00a0<\/span><\/p>\n

    \"p1100020\"<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    Pot\u0159ebn\u00e9 pom\u016fcky:<\/strong><\/p>\n

    a)\u00a0\u00a0\u00a0\u00a0\u00a0 fyziologick\u00fd (infuzn\u00ed) roztok\u00a0 0,9% chloridu sodn\u00e9ho v\u00a0 H2O\u00a0\u00a0 –\u00a0\u00a0 balen\u00ed 100-250 ml<\/p>\n

     <\/p>\n

    b)\u00a0\u00a0\u00a0\u00a0\u00a0 injek\u010dn\u00ed st\u0159\u00edka\u010dka 10ml<\/p>\n

    \"p1100032\"<\/p>\n

    c)\u00a0\u00a0\u00a0\u00a0\u00a0 injek\u010dn\u00ed jehla v\u011bt\u0161\u00edho pr\u016fm\u011bru cca 1,2 mm ( lep\u0161\u00ed v\u011bt\u0161\u00ed pr\u016fm\u011br z d\u016fvodu mo\u017en\u00e9ho ucp\u00e1v\u00e1n\u00ed )<\/p>\n

    \"p1100031\"<\/p>\n

    d)\u00a0\u00a0\u00a0\u00a0\u00a0 l\u00edh na desinfekci<\/p>\n

    e)\u00a0\u00a0\u00a0\u00a0\u00a0 kvasinkov\u00e1 kultura na ulo\u017een\u00ed<\/p>\n

    Postup:<\/strong><\/p>\n

      \n
    1. \u00a0P\u0159iprav\u00edme si kvasinky, kter\u00e9 chceme ulo\u017eit. Ukl\u00e1d\u00e1m kvasinky nakoupen\u00e9 jako tekut\u00e9, nebo p\u0159i rozkva\u0161ovac\u00ed f\u00e1zi odebran\u00e9 kvasinky p\u016fvodn\u011b zakoupen\u00e9 jako su\u0161en\u00e9, ze strippingu,\u00a0ale i kvasinky odebran\u00e9 p\u0159i kva\u0161en\u00ed ve f\u00e1zi b\u00edl\u00fdch krou\u017ek\u016f.<\/li>\n
    2. \u00a0N\u00e1sleduje nabr\u00e1n\u00ed vzorku v\u00a0mno\u017estv\u00ed cca 5-10ml do steriln\u00ed injek\u010dn\u00ed st\u0159\u00edka\u010dky s\u00a0nasazenou steriln\u00ed jehlou. U tekut\u00fdch kvasnic nab\u00edr\u00e1m 5ml, u p\u016fvodn\u011b such\u00fdch a z\u00a0kva\u0161en\u00ed nab\u00edr\u00e1m 10ml. P\u0159ed probodnut\u00edm s\u00e1\u010dku u tekut\u00fdch kvasnic je tento nutno ot\u0159\u00edt lihem s\u00a0koncentrac\u00ed 71%.<\/li>\n
    3. Z\u00a0krytu lahvi\u010dky fyziologick\u00e9ho roztoku odstran\u00edme ochranou \u010depi\u010dku event. p\u0159elepku (dle druhu v\u00fdrobce), preventivn\u011b takt\u00e9\u017e ot\u0159eme lihem a jehlou s\u00a0nabran\u00fdm vzorkem probodneme gumovou z\u00e1tku a vtla\u010d\u00edme kvasinky do roztoku. Jeden informa\u010dn\u00ed zdroj doporu\u010doval p\u0159ed touto operac\u00ed odebrat steriln\u011b cca10-20ml roztoku. Druh\u00fd informa\u010dn\u00ed zdroj doporu\u010doval do z\u00e1tky nasadit je\u0161t\u011b druhou injek\u010dn\u00ed st\u0159\u00edka\u010dku s\u00a0filtra\u010dn\u00ed steriln\u00ed vatou pro vyrovn\u00e1n\u00ed tlak\u016f. J\u00e1 jsem prozat\u00edm ob\u011b doporu\u010den\u00ed ignoroval. Roztok je nyn\u00ed jemn\u011b ml\u00e9\u010dn\u011b zakalen\u00fd. Kvasinky se postupn\u011b usad\u00ed na dn\u011b v\u00a0jemn\u00e9 vrstvi\u010dce<\/li>\n
    4. Injek\u010dn\u00ed st\u0159\u00edka\u010dku vyt\u00e1hneme a t\u00edm m\u00e1me p\u0159ipravenou kulturu pro ulo\u017een\u00ed. Nesm\u00edme zapomenout na pops\u00e1n\u00ed vzorku (datum ulo\u017een\u00ed a druh kultury). Vzorky je mo\u017eno pr\u00fd skladovat i p\u0159i pokojov\u00e9 teplot\u011b, ale j\u00e1 je m\u00e1m ve v\u00fd\u010depn\u00ed chladni\u010dce p\u0159i teplot\u011b cca 7\u00b0C.<\/li>\n
    5. P\u0159i pot\u0159eb\u011b rozmno\u017een\u00ed nastupuje obr\u00e1cen\u00fd postup. Lahvi\u010dku prot\u0159epeme, aby se kvasinky v\u00a0roztoku rovnom\u011brn\u011b rozpt\u00fdlily, steriln\u00ed jehlu se steriln\u00ed st\u0159\u00edka\u010dkou zabodneme do gumov\u00e9 z\u00e1tky p\u0159edt\u00edm ot\u0159en\u00e9 lihem, nasajeme 5ml roztoku, nasad\u00edme ochrann\u00fd kryt na jehlu a nech\u00e1me teplotu vzorku samovoln\u011b vyrovnat na pokojovou teplotu.<\/li>\n
    6. D\u00e1le postupujeme podle p\u0159\u00edsp\u011bvku propagace\u00a0 https:\/\/www.tourdebier.cz\/francek\/konecne-plantaznikem\/<\/a>\u00a0 . J\u00e1 pouze navy\u0161uji prvn\u00ed d\u00edl mladiny na cca 30-50ml.<\/li>\n<\/ol>\n

       <\/p>\n

      P\u0159i pou\u017eit\u00ed t\u00e9to metody jsem byl prozat\u00edm v\u017edy \u00fasp\u011b\u0161n\u00fd a na dodavatel\u00edch kvasnic se stal podstatn\u011b m\u00e9n\u011b z\u00e1visl\u00fdm. Prozat\u00edm m\u00e1m ve sv\u00e9 bance tyto kvasinky:<\/p>\n

      Orval\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a03068\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Safale US05<\/p>\n

      \"p1100024\"<\/p>\n

      w95\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0S189\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Hoeggarden\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Windsor<\/p>\n

      \"p1100026\"<\/p>\n

      P\u0159i v\u0161ech operac\u00edch se sna\u017e\u00edm dodr\u017eovat pravidla, kdy\u017e ne p\u0159\u00edmo pro steriln\u00ed, tak alespo\u0148 pro super\u010dist\u00e9 prost\u0159ed\u00ed a pr\u00e1ci v n\u011bm.<\/p>\n